FastQCFastQC Report
Sat 18 Mar 2023
SRR4895512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608123
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55360.9103421511766534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10730.17644456795746913No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA8800.14470756738357207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7460.12267255144107359No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC7350.12086370684877895No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA7150.11757489849915231No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT6190.10178861842094446No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG6190.10178861842094446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.0133.958228
GTGGTAT78400.0132.676736
AAGCAGT78450.0132.117661
GTATCAA78800.0132.094599
TGGTATC79050.0131.585777
AGTGGTA79300.0130.98945
AGCAGTG80350.0129.083162
CAGTGGT81050.0128.338754
GCAGTGG84050.0123.7435153
ATCAACG77200.027.52593210-14
CAACGCA77650.027.38495310-14
TCAACGC77800.027.35065810-14
AGAGTAC77350.027.30503715-19
CAGAGTA77650.027.19954315-19
TACATGG77800.027.11187620-24
GTACATG77900.027.0585920-24
CGCAGAG78550.026.96121215-19
AGTACAT78400.026.9227520-24
GAGTACA77550.026.69799220-24
GCAGAGT79350.026.68939415-19
Produced by FastQC (version 0.11.5)