FastQCFastQC Report
Sat 18 Mar 2023
SRR4895511_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895511_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845750
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGA100.007253358143.903938
GGTGCGA847350.0143.63221
GTGCGAA847350.0143.63222
TGCGAAT300250.0143.592393
TGCGAAG248850.0143.296743
GCGAATA87200.0143.16134
GCGAATG94800.0142.993154
TGCGAAA223600.0142.520233
GCGAAGT83600.0142.44084
GCGAATT91850.0142.102194
TGCGAAC78000.0141.874513
CGAATAG27900.0141.58295
CGAATAT30650.0141.321665
GCGAATC28500.0141.126834
GCGAAAT74250.0141.093694
CGAATTA25750.0140.27145
CGAAGTA27600.0140.25425
CGAAGAT25900.0140.014635
CGAATGG32950.0139.754945
CGAATGT32900.0139.748665
Produced by FastQC (version 0.11.5)