FastQCFastQC Report
Sat 18 Mar 2023
SRR4895511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845750
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65490.774342299733964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17980.21259237363287026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC17880.21140999113213127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT153900.0136.919981
GGTATCA153700.0136.736138
GTGGTAT154900.0135.86276
TGGTATC155300.0135.466427
GTATCAA155200.0135.414589
AGTGGTA157050.0133.91115
AGCAGTG157950.0133.226842
CAGTGGT158300.0133.035554
GCAGTGG161650.0130.132873
TCGTACG200.00629678228.78951350-54
TCAACGC153850.028.01293610-14
ATCAACG153550.027.97391910-14
AGAGTAC154350.027.90353815-19
CAACGCA155000.027.805110-14
CAGAGTA155600.027.7071315-19
GTACATG154950.027.68826520-24
TACATGG154650.027.66750320-24
CGCAGAG156350.027.5189815-19
GCAGAGT157000.027.46922915-19
AGTACAT156600.027.35975620-24
Produced by FastQC (version 0.11.5)