FastQCFastQC Report
Sat 18 Mar 2023
SRR4895510_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895510_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495924
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC1505500.0142.953371
AGAACAT491350.0142.933793
GAGAACA1506300.0142.877442
GAACATG158400.0142.145054
GAACATA144150.0141.375634
AGAACAG470850.0140.906893
GAACAGT148850.0140.39124
GAACATT151550.0140.16844
GAACAAT138750.0139.982394
GAACATC43950.0138.633164
AGAACAA421050.0137.053963
GAACAAG143800.0136.166994
GAACAGG162400.0136.163424
AGAACAC154550.0135.446123
AACATGT55450.0135.049135
AACATGA48100.0134.897555
AACATAG49450.0134.851585
GAACAGA136500.0134.1744
AACATGG58500.0133.54165
GAACACT37500.0133.320254
Produced by FastQC (version 0.11.5)