FastQCFastQC Report
Sat 18 Mar 2023
SRR4895510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495924
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147740.987617017976849No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18840.12594222701153265No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC18350.12266665953617965No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT16540.1105671143721205No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA16290.10889590647653223No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT14970.10007192878782611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214500.0137.109978
AAGCAGT214850.0136.668911
GTGGTAT216000.0136.091196
GTATCAA218200.0134.850979
TGGTATC218150.0134.81597
AGTGGTA218250.0134.688195
CAGTGGT220300.0133.500184
AGCAGTG220400.0133.325382
GCAGTGG225350.0130.332893
TCGTACG552.3283064E-1028.78442250-54
TCAACGC214100.028.11855110-14
ATCAACG214450.028.00554710-14
AGAGTAC215600.027.87063615-19
TACATGG215600.027.79184220-24
CAGAGTA216400.027.77425215-19
CAACGCA219900.027.42271610-14
GTACATG218750.027.39821820-24
AGTACAT219850.027.24148820-24
GAGTACA215400.027.22947520-24
GCAGAGT221400.027.08200315-19
Produced by FastQC (version 0.11.5)