FastQCFastQC Report
Sat 18 Mar 2023
SRR4895509_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895509_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790144
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA1791550.0143.814322
GCTCGGT1792600.0143.730091
TCGGTAA490900.0143.432393
TCGGTAG539850.0143.424263
TCGGTAT588550.0143.356123
CGGTATG188650.0142.63384
TCGGTAC178450.0142.559663
CGGTAAG156400.0142.550424
CGGTAGT177050.0142.467534
CGGTAAT167550.0142.298654
CGGTAGA149150.0142.242054
CGGTATA173500.0142.230184
CGGTAGG180950.0141.663864
CGGTATT188150.0140.909154
CGGTACG49100.0140.120014
CGGTATC45100.0139.143774
CGGTACT45900.0139.07044
CGGTAGC38600.0138.710514
CGGTAAA139650.0138.519884
CGGTACC47000.0137.959234
Produced by FastQC (version 0.11.5)