FastQCFastQC Report
Sat 18 Mar 2023
SRR4895509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790144
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169710.9480242930177685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG30790.17199733652711735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25050.1399328769082264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204800.0136.786568
GTGGTAT207650.0135.013176
GTATCAA208400.0134.423659
TGGTATC208600.0134.363777
AAGCAGT208900.0133.874071
AGTGGTA213100.0131.526445
CAGTGGT213650.0131.154164
AGCAGTG217000.0128.876922
GCAGTGG222350.0125.808393
ATCAACG203250.028.11131110-14
TCAACGC203700.028.08454510-14
AGAGTAC204350.027.86887615-19
TACATGG204950.027.7980420-24
CAGAGTA205750.027.6722515-19
GTACATG206450.027.60304520-24
CAACGCA209850.027.28206310-14
AGTACAT208700.027.23646520-24
GAGTACA204850.027.20013620-24
GCAGAGT212500.026.81357415-19
CGCAGAG212900.026.75643315-19
Produced by FastQC (version 0.11.5)