FastQCFastQC Report
Sat 18 Mar 2023
SRR4895508_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895508_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACACACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC30.10013351134846463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG252.986053E-8144.000024
CACACAT252.986053E-8144.000024
ACACTGT252.986053E-8144.000025
CGACACA2950.0144.01
ACTGTGT100.006879821144.07
ACACGGG100.006879821144.05
ACACGCG100.006879821144.05
ACACGAG100.006879821144.05
CACACTG357.2759576E-12144.04
CACACTA100.006879821144.04
ACAGATG100.006879821144.07
CACACGC151.1413853E-4144.04
CACACGA201.857683E-6144.04
CACACCG304.7475623E-10144.04
CACACAG304.7475623E-10144.04
CACACAC201.857683E-6144.04
CACACAA100.006879821144.04
ACAGAGT100.006879821144.07
CACCGTT100.006879821144.06
CACCGGT100.006879821144.06
Produced by FastQC (version 0.11.5)