FastQCFastQC Report
Sat 18 Mar 2023
SRR4895508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662.202937249666222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGCCAACTTCAGACGAAC200.6675567423230975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC160.5340453938584779No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGTGTGTGTAAACAGATAA130.43391188251001334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC120.4005340453938585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG100.33377837116154874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGGCGCAATAGATATAGTA90.30040053404539385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA90.30040053404539385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCACAGTGGCTCACGCCT60.20026702269692925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCCCTGCAGGAGGGCTGGG60.20026702269692925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCTGGTTTCCATCCACAA50.16688918558077437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA50.16688918558077437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA40.13351134846461948No Hit
CACAAAAGGCAGACAAGAACGAGGTTGCTGCGGAGGTGGCGAAACTCTTG40.13351134846461948No Hit
GAGGAGGCTACTGGCGTCGAGCCCAGTGCCTCAGAAGACTTCCTTTCATC30.10013351134846463No Hit
GGAGGTGCTACAGGGGCAACCTGGTTTCCATCCACAACTTCAATATTAAT30.10013351134846463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCTTCCTAGATCCCCTC30.10013351134846463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTAAAAAAAAAAAAAAAA30.10013351134846463No Hit
TGCCTGTCATCTCAGCACTTTGGGAGGCCGAGGCGGATGGATCACTTGAG30.10013351134846463No Hit
GTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGC30.10013351134846463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAGAGTTGAATCTCATTT30.10013351134846463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1150.0137.739145
AGCAGTG1150.0137.739142
GTGGTAT1150.0137.739146
CAGTGGT1150.0137.739144
GGTATCA1150.0137.739148
AAGCAGT1150.0137.739141
TGGTATC1200.0132.07
GTATCAA1200.0132.09
GCAGTGG1200.0132.03
ATGGGAA255.117988E-428.80000125-29
AACGCAG1150.028.80000110-14
ACATGGG1150.028.80000120-24
GTACATG1150.028.80000120-24
TCAACGC1150.028.80000110-14
TACATGG1150.028.80000120-24
AGAGTAC1150.028.80000115-19
CATGGGG403.084915E-728.80000125-29
CAGAGTA1200.027.615-19
CAACGCA1200.027.610-14
ACGCAGA1150.027.54782715-19
Produced by FastQC (version 0.11.5)