FastQCFastQC Report
Sat 18 Mar 2023
SRR4895507_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895507_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742308
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC28140.10261429423682533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC2747750.0143.608812
CCTCTAT2750300.0143.475661
TCTATCG871400.0143.06033
CTATCGA227050.0142.816824
TCTATCA711600.0142.610173
CTATCGG286500.0142.43774
CTATCGT296550.0142.390014
CTATCAG230450.0142.301974
CTATCTA210950.0142.15484
TCTATCT827500.0141.72063
CTATCAT244900.0141.720234
CTATCTG283900.0141.714774
TCTATCC362200.0141.31193
CTATCCG96150.0140.452134
TATCTAG72600.0139.930195
TATCGAT87350.0139.693535
CTATCAA195350.0139.585114
TATCGAG72150.0139.506595
TATCGGA75000.0139.00185
TATCGTA85400.0138.923635
Produced by FastQC (version 0.11.5)