FastQCFastQC Report
Sat 18 Mar 2023
SRR4895507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742308
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212350.7743477392036198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG98190.3580560608071741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC72120.26299015282017923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT63000.22973349456005673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT32090.11701821968940031No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC31960.11654416644665735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA414400.0136.541538
GTGGTAT415750.0136.15016
AAGCAGT417100.0135.749041
TGGTATC418200.0135.352487
GTATCAA418850.0135.142439
CAGTGGT425150.0133.190634
AGTGGTA425650.0132.915825
AGCAGTG428700.0131.975112
GCAGTGG435000.0130.096853
CGTGGTA1201.0063579E-535.9849975
TCAACGC414700.027.9862110-14
ATCAACG414950.027.89303610-14
AGAGTAC417450.027.73663715-19
CAGAGTA418850.027.63705815-19
CAACGCA421250.027.57155610-14
TACATGG419650.027.53874420-24
GTACATG422050.027.4026120-24
AGTACAT424750.027.1674120-24
CGCAGAG426800.027.12226315-19
GAGTACA416250.027.09959820-24
Produced by FastQC (version 0.11.5)