FastQCFastQC Report
Sat 18 Mar 2023
SRR4895506_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895506_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601596
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC8590.14278685363599491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCC602850.0143.591022
CCTAATC603500.0143.436391
TAATCCG224600.0143.32473
TAATCCT189650.0142.68733
ATCCGAT22050.0142.596125
ATCCTAG20050.0142.465915
AATCCGT78050.0142.33424
AATCCGA55800.0141.580344
TAATCCA122050.0141.484313
ATCCGGT27000.0141.23655
AATCCGG74950.0141.02144
AATCCTG64200.0140.987444
AATCCTA58050.0140.678744
TCCGATA6150.0140.391546
ATCCGTA21650.0140.245655
ATCCGTG24700.0140.114465
ATCCATG14500.0139.931645
AATCCAT41750.0139.765264
ATCCTAT21250.0139.499655
ATCCGAG16250.0139.473625
Produced by FastQC (version 0.11.5)