FastQCFastQC Report
Sat 18 Mar 2023
SRR4895506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601596
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36260.6027300713435595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15090.2508327847924521No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12320.20478859566885418No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA6510.10821215566592862No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC6120.10172939979654119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT112700.0137.436691
GGTATCA112800.0137.171148
GTGGTAT113150.0136.874056
TGGTATC113500.0136.451977
GTATCAA113400.0136.318459
AGTGGTA114550.0135.138385
CAGTGGT115550.0134.2184
AGCAGTG115750.0133.877462
GCAGTGG116000.0133.713063
TCAACGC112650.028.13516810-14
ATCAACG112600.028.05818410-14
AGAGTAC113150.027.90907715-19
TACATGG113100.027.81335320-24
CAGAGTA113850.027.73747815-19
CAACGCA114450.027.70525210-14
GTACATG113750.027.6797320-24
AGTACAT114200.027.55805220-24
GCAGAGT115700.027.29396615-19
CGCAGAG116200.027.25084315-19
GAGTACA113150.027.22849820-24
Produced by FastQC (version 0.11.5)