FastQCFastQC Report
Sat 18 Mar 2023
SRR4895505_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895505_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080540
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACAACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC21080.10131984965441664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC2086150.0143.513582
CCGACAA2088900.0143.324651
GACAACT631000.0143.196353
GACAACG661700.0142.989933
ACAACGT222750.0141.900624
ACAACTG211000.0141.31174
GACAACA557500.0141.012343
ACAACTA177550.0139.850854
CAACGTA68000.0139.776695
CAACGTG73000.0139.467945
CAACGGT74650.0139.373215
CAACGAT64650.0139.006385
CAACTAG59850.0138.613655
ACAACGA180900.0138.533784
ACAACGG222400.0138.532824
CAACGTT77550.0138.243685
CAACTAT60300.0137.937185
CAACTGT74750.0137.935425
GACAACC259950.0137.730883
CAACTGA57550.0136.65195
Produced by FastQC (version 0.11.5)