FastQCFastQC Report
Sat 18 Mar 2023
SRR4895505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2080540
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167180.8035413882934238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG41350.19874647927941783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32440.155921058955848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT309300.0136.075871
GGTATCA308300.0135.981058
GTGGTAT310150.0135.35566
GTATCAA311550.0134.747369
TGGTATC312400.0134.35777
AGTGGTA315900.0132.84635
AGCAGTG318150.0132.177472
CAGTGGT320400.0131.182654
GCAGTGG323650.0129.886813
TCAACGC307750.027.93236710-14
ATCAACG308150.027.8120310-14
AGAGTAC310350.027.63886615-19
GTACATG311050.027.5314520-24
TACATGG311050.027.47590420-24
CAGAGTA312450.027.43467315-19
CAACGCA314000.027.39014410-14
AGTACAT313600.027.22953220-24
GAGTACA309500.027.01341620-24
GCAGAGT317300.027.00625615-19
CGCAGAG317700.026.99037615-19
Produced by FastQC (version 0.11.5)