FastQCFastQC Report
Sat 18 Mar 2023
SRR4895504_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895504_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216312
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC13310.10942915962351765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG404150.0143.308853
CATCCTC1222200.0143.255082
CCATCCT1223350.0143.12041
ATCCTCA312000.0143.004153
ATCCTCT355950.0141.948143
TCCTCGA104800.0141.68364
TCCTCGG137650.0140.603524
CCTCGTA40700.0139.88265
TCCTCAG105700.0139.864354
TCCTCGT137150.0139.699174
CCTCGAT33750.0139.471535
CCTCGAG38200.0138.674325
TCCTCTG124150.0138.616354
CCTCTGA33350.0138.338735
TCCTCAT107450.0138.122314
CCTCAGT36050.0137.960375
TCCTCTA98450.0137.662664
ATCCTCC160350.0137.35173
CCTCGGT46450.0137.132115
CTCGAAT10350.0136.995866
Produced by FastQC (version 0.11.5)