FastQCFastQC Report
Sat 18 Mar 2023
SRR4895504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1216312
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102220.8404093686488334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG55050.4525976887509126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38060.3129131341300587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC26770.22009155545616588No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGAGAGCAAGCCTCTGAC25340.20833470359578793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT215850.0130.517681
GGTATCA216550.0130.009068
TGGTATC217850.0129.332377
GTGGTAT217850.0129.233236
GTATCAA218900.0128.711999
CAGTGGT220100.0127.879434
AGTGGTA221400.0127.1610645
AGCAGTG223000.0126.300622
GCAGTGG232750.0120.978883
CGTGGTA850.002839681533.8754465
TACATGG214750.026.57725520-24
TCAACGC216550.026.54697810-14
ATCAACG216150.026.5428210-14
GTACATG215850.026.46182620-24
AGAGTAC216300.026.39794715-19
AGTACAT216900.026.2872620-24
CAGAGTA217550.026.25950615-19
ACATGGG217600.026.06374220-24
CAACGCA222400.025.89400110-14
GCAGAGT221350.025.82170715-19
Produced by FastQC (version 0.11.5)