FastQCFastQC Report
Sat 18 Mar 2023
SRR4895503_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895503_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872573
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC29600.103043508380814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC2883100.0143.374312
CACCTTA2884100.0143.32461
CCTTACG823650.0143.228263
CTTACGA220700.0142.564244
CTTACGT288450.0142.304494
CCTTACA804550.0141.90663
CTTACGG255500.0141.394594
CCTTACT933450.0141.21083
CTTACTG284600.0141.119954
CTTACTA261350.0140.927614
CTTACAG243600.0140.533754
TTACGAT87550.0139.627415
TTACGTA88050.0139.079685
TTACGTG89900.0139.018835
CTTACGC67150.0138.972054
TTACGGT95250.0138.764275
TTACGAG64300.0138.641685
CTTACCG89200.0138.173894
CCTTACC358950.0137.646943
TTACGGA72450.0137.34645
Produced by FastQC (version 0.11.5)