FastQCFastQC Report
Sat 18 Mar 2023
SRR4895503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872573
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262730.9146155728679481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG115760.4029836665595618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC87040.30300361383331254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA402800.0136.843618
GTGGTAT406850.0135.569876
AAGCAGT407550.0135.318191
GTATCAA407350.0135.297459
TGGTATC408100.0135.20757
AGCAGTG418750.0131.681732
AGTGGTA419800.0131.456385
CAGTGGT421200.0131.104864
GCAGTGG429550.0128.437963
TCAACGC401900.028.05317910-14
ATCAACG402700.027.91881210-14
AGAGTAC406000.027.6821215-19
CAGAGTA407300.027.57609615-19
GTACATG408450.027.45142420-24
TACATGG410400.027.30344820-24
AGTACAT412450.027.16075720-24
GAGTACA403950.027.15845920-24
CAACGCA417350.027.02156610-14
GCAGAGT416150.027.00002915-19
TATCAAC414000.026.67698910-14
Produced by FastQC (version 0.11.5)