FastQCFastQC Report
Sat 18 Mar 2023
SRR4895502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850057
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGCT572050.0143.322313
AAGGAGC1860350.0143.116712
AGGAGCG569850.0142.10783
CAAGGAG1874900.0142.006071
GGAGCTA162100.0142.004734
AGGAGCA487050.0141.579223
GGAGCAT162550.0141.257464
GGAGCGT200150.0140.96554
GGAGCTG180350.0140.482244
GAGCTAT58850.0139.756295
GAGCTAG53800.0139.232325
GGAGCTT186000.0139.039034
GGAGCGA146500.0139.00184
GAGCGAT54200.0139.001335
AGGAGCC248400.0138.177833
GAGCTGA48650.0138.145145
GAGCGTA56000.0138.131295
GAGCTAA46700.0137.75025
GAGCTTA51300.0137.741795
GAGCATA47250.0137.517355
Produced by FastQC (version 0.11.5)