FastQCFastQC Report
Sat 18 Mar 2023
SRR4895502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850057
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166720.9011614236750544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27810.1503196928527067No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA24610.13302292848274405No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC23820.12875278977890953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22210.12005035520527205No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT21840.11805041682499513No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC21810.11788825965902672No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA20680.11178033974088367No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT20300.10972634897195059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19370.1046994768269302No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA18930.10232117172606034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227800.0137.279138
GTGGTAT229500.0136.356346
AAGCAGT230250.0135.858781
TGGTATC230900.0135.529577
GTATCAA231150.0135.445279
AGTGGTA235850.0132.654575
AGCAGTG236450.0132.205062
CAGTGGT237900.0131.481224
GCAGTGG246600.0126.8219153
TCAACGC227950.028.10081110-14
ATCAACG227450.028.09929810-14
AGAGTAC227950.027.95557215-19
CAGAGTA229350.027.78492715-19
TACATGG229050.027.7818320-24
GTACATG229800.027.7287520-24
AGTACAT231900.027.4279820-24
CAACGCA235050.027.25199110-14
GCAGAGT234300.027.24092515-19
GAGTACA228900.027.19623420-24
CGCAGAG237100.026.90708515-19
Produced by FastQC (version 0.11.5)