FastQCFastQC Report
Sat 18 Mar 2023
SRR4895501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338385
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT1341850.0143.524461
TGGCTCA332000.0143.44473
TTGGCTC1343350.0143.364212
TGGCTCG389100.0143.104693
TGGCTCT418250.0142.747223
GGCTCAT114100.0142.512514
GGCTCTA117600.0142.24794
GGCTCAG106850.0141.542984
GGCTCGA98400.0141.413734
GCTCTAG36700.0141.351175
GGCTCGT134900.0141.233024
GGCTCTG136750.0141.111254
GCTCATA33750.0140.91525
GCTCATG39200.0140.779535
TGGCTCC210250.0140.683013
GCTCGTA36900.0140.195075
GCTCAGT38100.0139.934055
GCTCTAA35200.0139.811745
GCTCGAT38000.0139.734285
GGCTCGG128800.0139.207434
Produced by FastQC (version 0.11.5)