FastQCFastQC Report
Sat 18 Mar 2023
SRR4895501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338385
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117350.8768030125860646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47410.35423290009974706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36740.27450995042532605No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18390.13740440904522988No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC17580.13135233882627195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT16690.12470253327704658No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA15470.11558706949046799No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT15170.11334556200196506No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT15060.11252367592284732No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA13450.1004942524012149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT205850.0136.967941
GGTATCA207150.0136.19148
GTGGTAT208300.0135.439516
GTATCAA208650.0135.108839
TGGTATC209650.0134.533047
AGTGGTA211950.0133.10715
AGCAGTG212100.0132.931852
CAGTGGT214950.0131.349814
GCAGTGG217500.0129.598393
TCAACGC207050.027.87015510-14
AGAGTAC207700.027.76214415-19
ATCAACG207500.027.74034510-14
CAACGCA208650.027.6840310-14
CAGAGTA208250.027.66117915-19
TACATGG208000.027.58275620-24
GTACATG209200.027.47271320-24
GCAGAGT211450.027.24256315-19
CGCAGAG211700.027.18319515-19
AGTACAT211900.027.15663320-24
GAGTACA208200.027.02380620-24
Produced by FastQC (version 0.11.5)