FastQCFastQC Report
Sat 18 Mar 2023
SRR4895500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506340
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC6330.1250148121815381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC508200.0143.393051
TCATTCC508500.0143.308462
CATTCCG170600.0142.918753
CATTCCT156700.0142.185993
ATTCCGT59650.0142.121464
ATTCCGA45050.0141.534974
ATTCCGG52350.0140.906834
CATTCCA120550.0140.826893
ATTCCTA49850.0139.455874
ATTCCTG46600.0139.452624
TTCCGAG13350.0139.079565
TCCGGAT6300.0138.219626
TCCGATA6000.0137.934046
TTCCGAT19000.0137.87095
TTCCGTA17700.0137.42585
TCCGGTA6450.0137.23676
ATTCCAG38800.0136.326574
CATTCCC67900.0135.98213
ATTCCGC15850.0135.758424
ATTCCAT43950.0135.743944
Produced by FastQC (version 0.11.5)