FastQCFastQC Report
Sat 18 Mar 2023
SRR4895499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132155
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA2133700.0143.777482
GCTAACG2133750.0143.77411
TAACGAT682650.0143.629063
TAACGAG667150.0143.42913
TAACGAC230350.0143.101213
TAACGAA560250.0142.931763
AACGATG229200.0142.846194
AACGATA193400.0142.208084
ACGATGA66000.0141.919975
AACGAGT200200.0141.761864
AACGATT210350.0141.385354
AACGAAT187300.0141.231744
ACGATAG67450.0140.78895
ACGATTG75150.0140.531435
ACGATGT78350.0140.025565
ACGAGTA60850.0139.98055
AACGAGG240550.0139.874484
ACGATTA58750.0139.841055
AACGATC57850.0139.405124
ACGATGG80000.0139.385685
Produced by FastQC (version 0.11.5)