FastQCFastQC Report
Sat 18 Mar 2023
SRR4895499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132155
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169240.7937509233615756No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA27150.12733595822067345No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC24290.1139222992699874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23960.1123745693910621No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA22000.10318199192835419No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT21980.10308819011751022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA332000.0136.426398
AAGCAGT332850.0136.003191
GTGGTAT333150.0135.912256
TGGTATC333850.0135.691967
GTATCAA335050.0135.205969
AGTGGTA338000.0133.898165
CAGTGGT341700.0132.553594
AGCAGTG344200.0131.476652
GCAGTGG346750.0130.572083
CGTGGTA1703.193527E-629.6318555
TCAACGC331700.028.07362710-14
ATCAACG331250.028.02486610-14
AGAGTAC333200.027.81805215-19
CAGAGTA334350.027.73528515-19
TACATGG334550.027.69938920-24
GTACATG334600.027.67373520-24
AGTACAT336700.027.44554920-24
CAACGCA339950.027.40079710-14
GCAGAGT340400.027.23388315-19
GAGTACA333400.027.0176920-24
Produced by FastQC (version 0.11.5)