FastQCFastQC Report
Sat 18 Mar 2023
SRR4895498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2601623
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC2604200.0143.773932
ATAGCGA2604700.0143.746321
AGCGACT798600.0143.449053
AGCGACG773750.0143.164293
GCGACTA230450.0143.012074
GCGACTG246250.0142.865634
AGCGACA704750.0142.84553
GCGACGT265450.0142.806184
GCGACAT239250.0142.744614
GCGACTT262250.0142.243844
GCGACGA206300.0141.510834
CGACGTA80650.0141.508585
AGCGACC342750.0141.25133
CGACTGA70050.0141.246775
CGACTAT81150.0141.168725
CGACATG77650.0141.137485
CGACTTA77500.0140.667865
CGACTAA70450.0140.546945
CGACTAG75900.0140.504555
GCGACGG251550.0140.341844
Produced by FastQC (version 0.11.5)