FastQCFastQC Report
Sat 18 Mar 2023
SRR4895497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976787
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC1979600.0143.702852
AGGCTAA1980700.0143.623031
GCTAACT602450.0143.611423
GCTAACG600750.0143.586643
GCTAACA521900.0143.262043
CTAACGT202050.0143.090944
GCTAACC258850.0142.659123
CTAACTG197500.0142.562014
CTAACGG192400.0142.30194
CTAACGA160050.0142.201634
CTAACTA166250.0141.529564
CTAACTT195600.0141.077444
TAACGTG63850.0140.64195
CTAACAG171500.0140.38574
CTAACAT180700.0140.246574
TAACTAG55400.0139.364145
CTAACCG69850.0139.274414
TAACGAT58250.0139.215965
TAACGTA61800.0139.019885
CTAACTC52300.0138.406774
Produced by FastQC (version 0.11.5)