FastQCFastQC Report
Sat 18 Mar 2023
SRR4895497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976787
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146610.7416580542061436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA45630.23082911815992313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35970.1819619412713661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC26220.13263948012608337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAT25190.1274290047435561No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA22310.11285990852833411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21920.1108870100825228No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21890.11073524866361424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303150.0136.678288
GTGGTAT303800.0136.504296
AAGCAGT304400.0136.401751
TGGTATC306450.0135.46487
GTATCAA306400.0135.111079
AGTGGTA308750.0134.385735
CAGTGGT311400.0133.357684
AGCAGTG312850.0132.648532
GCAGTGG320350.0129.677833
TCAACGC303100.028.02402310-14
ATCAACG302900.027.9617410-14
AGAGTAC303300.027.8924915-19
CAGAGTA305100.027.74208615-19
TACATGG304200.027.7248320-24
GTACATG305550.027.63531920-24
AGTACAT307250.027.45429620-24
CAACGCA312700.027.20050210-14
GAGTACA303300.027.15677820-24
GCAGAGT311550.027.14926115-19
ACATGGG309050.027.01022120-24
Produced by FastQC (version 0.11.5)