FastQCFastQC Report
Sat 18 Mar 2023
SRR4895496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944895
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCATG1947650.0143.691861
GCCATGC1948800.0143.607072
CCATGCG615800.0143.314533
CCATGCA477550.0142.994773
CCATGCT540100.0142.95293
CATGCGA163150.0142.399324
CATGCGT201700.0142.32924
CATGCGG200000.0141.920144
CATGCTG182550.0141.691574
CATGCAG154250.0141.613144
CATGCAT157200.0141.518744
CATGCTA150650.0141.176454
CATGCTT169000.0140.450274
CATGCCG91400.0140.356374
CCATGCC329050.0140.137823
CATGCAA135900.0139.875084
CATGCGC56750.0139.714894
ATGCGAT58050.0139.064935
ATGCTAG48600.0139.013285
ATGCGGT61750.0139.005025
Produced by FastQC (version 0.11.5)