FastQCFastQC Report
Sat 18 Mar 2023
SRR4895496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944895
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141500.7275457029813949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG57180.29400044732491987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38220.19651446479115842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC26120.1343003092711946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT23020.11836114546029478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22630.11635589581956868No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20390.10483856454975718No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA20140.10355314811339429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA292050.0135.950998
GTGGTAT292650.0135.893596
AAGCAGT294800.0135.327841
TGGTATC295200.0134.695337
GTATCAA295550.0134.365369
AGTGGTA300650.0132.421225
CAGTGGT302350.0131.867084
AGCAGTG305850.0130.367972
GCAGTGG309800.0128.636053
TCAACGC291400.027.95224810-14
ATCAACG291150.027.91692410-14
AGAGTAC292500.027.75898215-19
CAGAGTA293650.027.65517215-19
TACATGG293450.027.62574820-24
GTACATG295800.027.40627520-24
AGTACAT297900.027.22757320-24
CAACGCA299900.027.16000410-14
GAGTACA292900.027.07304820-24
GCAGAGT300100.027.05119315-19
CGCAGAG302450.026.89335615-19
Produced by FastQC (version 0.11.5)