FastQCFastQC Report
Sat 18 Mar 2023
SRR4895495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469953
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT1472800.0143.61431
GCACCTC1473150.0143.580182
CACCTCG461650.0143.05123
ACCTCGT150350.0142.505024
ACCTCGA122750.0142.427464
CACCTCT441050.0142.1313
CACCTCA385250.0142.081253
ACCTCGG154550.0141.518594
ACCTCAT130450.0141.466054
ACCTCAG121700.0141.35074
ACCTCTG140150.0141.06934
CCTCGTA45950.0140.761255
ACCTCTA127800.0140.683884
CCTCGAT41950.0139.433625
CCTCGAG38950.0138.351325
CCTCAGT41450.0138.33845
CCTCGGT53800.0138.276115
CCTCGAA38950.0137.243035
CCTCGTG48300.0137.189685
CCTCTAG43700.0136.9785
Produced by FastQC (version 0.11.5)