FastQCFastQC Report
Sat 18 Mar 2023
SRR4895495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469953
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121040.8234276878240324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36390.24755893555780356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32140.21864644651903836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA28990.1972171899373653No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA21370.1453787978255087No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20390.13871191800009933No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT19330.13150080308690143No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA18200.12381348247188856No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC16070.10932322325951918No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA15950.1085068706278364No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT15580.1059897833468145No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG15060.1024522552761891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191750.0134.701668
AAGCAGT193300.0133.67611
GTGGTAT193250.0133.618876
TGGTATC193600.0133.37737
GTATCAA193900.0133.208079
CAGTGGT198750.0129.848824
AGTGGTA199150.0129.47965
AGCAGTG200450.0128.907912
GCAGTGG203850.0126.651913
ATCAACG191450.027.60655210-14
TCAACGC192000.027.57994510-14
AGAGTAC190850.027.54250715-19
TACATGG191850.027.36025820-24
CAGAGTA193450.027.20953215-19
GTACATG192850.027.18852220-24
AGTACAT193800.027.04781720-24
CAACGCA196300.027.02712610-14
GAGTACA190600.026.89765420-24
GCAGAGT196800.026.7756215-19
CGCAGAG198900.026.55804815-19
Produced by FastQC (version 0.11.5)