FastQCFastQC Report
Sat 18 Mar 2023
SRR4895494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708208
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA53080.7494973228204144No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA48990.6917459277500395No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA43920.6201567900955651No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGTTGATA37520.5297878589340985No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA27400.38689198653502926No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA25720.36317014210514426No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACACTGCGTTGATAC20980.296240652463683No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGCGGATA13700.19344599326751463No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC11070.1563100106183494No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACTCTGCGTTGATAC9950.14049544766509275No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGTGGATA8630.12185685561304023No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGGACGCTGCGTTGATA7970.11253755958701397No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCACGGACGCTGCGTTGATA7770.10971353048821815No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACTCTGCGTTGATA7690.10858391884869982No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGGACGCTGCGTTGATA7600.1073131057542417No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACGCTGCGTTGATA7490.10575988974990398No Hit
AGATGTACTCTGCGTTGATACCACAGCTTCCCACGGACTCTGCGTTGATA7100.10025303300725211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC708550.0143.830582
AGATGTA709400.0143.658231
TGTACGT59650.0142.695434
TGTACGA45700.0142.642124
GTACGTA17000.0142.631935
TGTACGG65650.0142.148094
TGTACAG50300.0141.469914
TGTACTA45500.0141.371524
TGTACTG56100.0140.695284
GTACGAT14650.0140.463735
GTACGTG19500.0140.211875
TGTACAT48700.0140.208084
GTACTAA13300.0140.114765
TGTACCT18900.0140.094734
ATGTACA149150.0139.849443
GTACAGT15250.0139.183585
GTACGTT18200.0138.76235
GTACGGT20750.0138.353625
ATGTACG190600.0138.239213
TACGATG4850.0137.967566
Produced by FastQC (version 0.11.5)