FastQCFastQC Report
Sat 18 Mar 2023
SRR4895494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708208
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8200111.578660506517858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA228843.231254094842193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36480.5151029076203602No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA20840.2942638320945259No Hit
AAGCAGTGGTATCAACGCAGAGTACATCTAGATCGGAAGAGCACACGTCT12340.17424259539570297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG11680.1649232993696767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG11650.16449969500485734No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA9730.13738901565641734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8220.11606759596050878No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7950.11225515667713441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT576300.050.2482381
GTATCAA612150.047.297219
GGTATCA617050.046.8866358
AGTGGTA632000.045.731985
TGGTATC644500.044.945517
GTGGTAT644600.044.9050456
AGCAGTG653250.044.3071672
CAGTGGT657250.043.9750634
GCAGTGG665400.043.4656753
TTCGCGA300.001558907823.993616105-109
CGACCTA709.413634E-820.57176845-49
ATGGGGG18650.019.93448825-29
ATGGGAG19950.019.71895825-29
CGGCTAG1250.019.581282115-119
TACGCAC853.56722E-818.6271850-54
ATGGGGT6750.018.57289925-29
AAACCGC2650.018.47786585-89
AACGCGA803.8381404E-718.00029645-49
CGGGTAG2650.017.929585115-119
GATAGGT652.342682E-517.72136730-34
Produced by FastQC (version 0.11.5)