FastQCFastQC Report
Sat 18 Mar 2023
SRR4895493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798262
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1801300.0143.635512
ACAGATT1803600.0143.452351
AGATTCG555100.0143.334813
AGATTCA478950.0142.947883
AGATTCT556150.0142.495063
GATTCGA148650.0142.330494
AGATTCC221400.0142.059523
GATTCGG177350.0141.932074
GATTCAG153200.0141.625154
GATTCGT195100.0141.408634
GATTCAT164300.0141.383354
GATTCTG175600.0140.069124
GATTCGC40350.0139.956794
GATTCAA139850.0139.918214
ATTCGAG47200.0139.30675
GATTCCG56300.0138.767974
ATTCGTA56100.0138.749745
GATTCTA159000.0138.675954
ATTCGGA48350.0137.927575
ATTCGGT65300.0137.379235
Produced by FastQC (version 0.11.5)