FastQCFastQC Report
Sat 18 Mar 2023
SRR4895493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798262
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167010.9287300738157176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG56190.3124683722394178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT30550.1698862568413279No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25310.14074701016870733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT23850.13262805975992376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC22420.12467593709926586No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18360.10209858185292243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT282200.0135.913481
GGTATCA282900.0135.60738
GTGGTAT283450.0135.242636
TGGTATC284750.0134.751547
GTATCAA285800.0134.281669
AGTGGTA289250.0132.505875
AGCAGTG291150.0131.686042
CAGTGGT291950.0131.452994
GCAGTGG298350.0128.556353
TCAACGC283050.027.95116810-14
ATCAACG283000.027.86456110-14
AGAGTAC284700.027.69312115-19
CAGAGTA285550.027.62580915-19
TACATGG286050.027.50328620-24
GCAGAGT289350.027.25305415-19
GTACATG289850.027.15264520-24
CAACGCA291550.027.141210-14
AGTACAT292250.026.91488620-24
CGCAGAG293150.026.89978215-19
GAGTACA285150.026.87320520-24
Produced by FastQC (version 0.11.5)