FastQCFastQC Report
Sat 18 Mar 2023
SRR4895492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911384
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC11010.12080528075981145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC913900.0143.495561
ACGACCG321300.0143.404433
CACGACC915250.0143.283922
CGACCGT102800.0143.057224
ACGACCT262900.0142.939213
CGACCTA77050.0142.589774
CGACCGA82500.0142.41454
ACGACCA208800.0142.174163
CGACCAT66750.0141.417954
GACCGTA28800.0141.149055
CGACCTG93650.0141.054474
CGACCTT77150.0140.633044
CGACCAG77300.0140.360144
GACCTAG25800.0139.992895
ACGACCC127550.0139.553653
GACCGTG35050.0139.381045
CGACCGG114350.0139.303964
GACCGAT30200.0139.132285
GACCTGA27150.0138.8625
GACCTAA25150.0138.747675
Produced by FastQC (version 0.11.5)