FastQCFastQC Report
Sat 18 Mar 2023
SRR4895492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911384
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76380.8380660621647955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18670.20485327809134238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC17070.18729756063305916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15670.17193630785706135No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14010.1537222509940925No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA12630.13858044468632322No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC12270.1346304082582095No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG10600.11630662816112637No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT10300.11301493113769828No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA10240.11235659173301266No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT9890.1085162785390132No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG9400.10313984006741395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120350.0136.157618
GTGGTAT121000.0135.307246
TGGTATC122400.0133.877187
GTATCAA122550.0133.772059
AAGCAGT122550.0133.73531
CAGTGGT126100.0130.177264
AGTGGTA126400.0129.69755
AGCAGTG126600.0129.343292
GCAGTGG128400.0127.4739763
ATCAACG120550.027.7355610-14
TCAACGC121000.027.66809810-14
AGAGTAC121150.027.45653715-19
TACATGG121550.027.33725720-24
GTACATG121600.027.32601720-24
CAGAGTA121850.027.29880715-19
CAACGCA122950.027.26439710-14
AGTACAT122300.027.11075220-24
GAGTACA120300.026.95112220-24
CGCAGAG124100.026.89665215-19
GCAGAGT124050.026.87268315-19
Produced by FastQC (version 0.11.5)