FastQCFastQC Report
Sat 18 Mar 2023
SRR4895491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834822
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC837250.0143.49492
AATGTTG840550.0142.931531
TGTTGCA212650.0142.867033
TGTTGCG256200.0142.539783
GTTGCAT71150.0142.500124
GTTGCGA65050.0142.367364
GTTGCAG64000.0141.55494
GTTGCTA67300.0141.456854
TGTTGCT251050.0140.906433
GTTGCAA65600.0140.405884
GTTGCTG82700.0139.913534
GTTGCGG87700.0139.895574
GTTGCGT85900.0139.392474
TTGCAGT21150.0139.152855
TGTTGCC129050.0138.451553
TTGCGAT24500.0138.335625
TTGCATA21500.0137.891655
TTGCGGA23850.0137.580115
TTGCGAG22000.0137.37445
TTGCTAG23350.0137.136265
Produced by FastQC (version 0.11.5)