FastQCFastQC Report
Sat 18 Mar 2023
SRR4895491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834822
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61380.7352465555531598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22580.2704768202083797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13880.1662629877985966No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC11470.13739455836094402No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA11440.13703520031815164No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA10220.12242130657792918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10000.11978601426411858No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC9810.11751007999310033No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT9620.11523414572208207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT8710.10433361842404729No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG8660.1037346883527267No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT8650.10361490233846257No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA8350.10002132191053902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115300.0133.903178
AAGCAGT115500.0133.290951
GTGGTAT116500.0132.462136
GTATCAA116750.0132.301779
TGGTATC117050.0131.83977
AGCAGTG118900.0129.418872
CAGTGGT119600.0128.7884
AGTGGTA119800.0128.633075
GCAGTGG121750.0126.330213
ATCAACG115600.027.29641210-14
TCAACGC116150.027.20433810-14
AGAGTAC115550.027.14626915-19
CAGAGTA115400.027.1441315-19
CAACGCA117050.027.00746310-14
GTACATG115900.026.99364920-24
CGCAGAG116150.026.9812515-19
TACATGG116400.026.85295920-24
AGTACAT116550.026.84310320-24
GCAGAGT117150.026.72635815-19
GAGTACA115050.026.51731920-24
Produced by FastQC (version 0.11.5)