FastQCFastQC Report
Sat 18 Mar 2023
SRR4895490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057092
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC2059800.0143.704732
AATCCGT2060950.0143.624561
TCCGTCA545350.0143.078873
TCCGTCG622500.0143.018433
TCCGTCT624350.0142.525513
CCGTCGA166500.0142.470844
CCGTCTA173900.0142.117784
CCGTCAG165650.0141.768594
CCGTCAT191250.0141.602024
CCGTCTG191700.0141.569874
CCGTCGT220300.0141.545384
CGTCGTA64950.0140.795155
CGTCGAA52050.0140.579335
CGTCAGT60700.0140.577975
CGTCTAG55600.0140.532355
CCGTCGG195050.0140.429464
CGTCATA58300.0140.317935
CGTCTAA52800.0140.217675
CGTCGAG49250.0140.098545
CGTCGGA55350.0139.86725
Produced by FastQC (version 0.11.5)