FastQCFastQC Report
Sat 18 Mar 2023
SRR4895490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057092
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154080.7490185174022358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG37510.18234478574609206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG27160.13203104187853532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC26110.1269267490224064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21240.10325255263255119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271600.0135.324588
GTGGTAT272400.0134.874316
AAGCAGT274450.0134.128811
GTATCAA275100.0133.576749
TGGTATC276050.0133.247397
AGTGGTA277750.0132.302295
CAGTGGT281450.0130.818734
AGCAGTG285600.0128.917562
GCAGTGG288350.0127.7130363
TCAACGC270200.027.96158810-14
ATCAACG270000.027.90233610-14
AGAGTAC272500.027.59921615-19
CAGAGTA274050.027.4536215-19
GTACATG274150.027.39144720-24
TACATGG275000.027.27013620-24
CAACGCA277350.027.24074710-14
AGTACAT276650.027.11269420-24
CGCAGAG280550.026.83807615-19
GAGTACA273150.026.77491820-24
GCAGAGT281550.026.74786615-19
Produced by FastQC (version 0.11.5)