FastQCFastQC Report
Sat 18 Mar 2023
SRR4895489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446402
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACACT445600.0142.87123
AGGACAC1456950.0142.86262
GACACTG134600.0142.621674
GACACGT141750.0142.483344
GACACAT140500.0142.41954
AAGGACA1462350.0142.335041
GGACACG420200.0142.260793
GACACTT151900.0141.678334
GGACACA408700.0141.545553
GACACTA130500.0141.037574
GACACGA115350.0140.41154
GACACAG126150.0139.227584
GACACGG134950.0138.946084
ACACATG47050.0138.24635
ACACTGT50800.0137.389255
ACACTTG54000.0136.842645
ACACGAT45000.0136.70945
ACACGTA44850.0136.685325
GGACACC196500.0136.654463
ACACGGT48000.0136.55955
Produced by FastQC (version 0.11.5)