FastQCFastQC Report
Sat 18 Mar 2023
SRR4895489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446402
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115290.7970813093455346No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA26520.18335151638341207No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC25470.17609212376642178No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA24170.16710430433586237No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT22890.1582547590503885No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT22020.15223983373916797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21000.14518785233980594No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC20500.1417309987126677No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG19250.1330888646448221No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA19010.13142957490379575No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG17950.1241010452142627No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA17860.12347881156137783No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT17540.12126642524000934No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG16860.11656510430710135No Hit
AGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTC16410.11345393604267694No Hit
AGACGCTTTCAGTGGGTTGACGGCAGCCGCTGGAACTTTGCGTACTGGGC16340.11296997653487757No Hit
CCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTAC16160.11172550922910782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC16060.11103413850368016No Hit
ATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCTC15870.10972053412536763No Hit
CATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCT15870.10972053412536763No Hit
AGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGC15410.10654022878840046No Hit
TTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCT15350.10612540635314388No Hit
GTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCC15300.10577972099043004No Hit
CCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCC15120.10453525368466028No Hit
GAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTC15050.10405129417686092No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG14970.10349819759651882No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT14910.10308337516126223No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG14630.10114753713006482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161000.0137.728268
GTGGTAT161550.0137.170266
AAGCAGT162500.0136.24771
TGGTATC163200.0136.048037
GTATCAA164250.0135.003049
AGTGGTA165200.0134.270255
CAGTGGT166500.0133.265124
AGCAGTG166750.0132.775122
GCAGTGG172500.0128.3913
ATCAACG160350.028.1510310-14
TCAACGC160750.028.13470810-14
AGAGTAC160900.028.02018215-19
CAGAGTA162350.027.76992415-19
TACATGG161950.027.73779720-24
CAACGCA162950.027.72835710-14
GTACATG162750.027.6279920-24
AGTACAT163700.027.45006620-24
GAGTACA160800.027.43475220-24
CGCAGAG165150.027.29910315-19
AACGCAG167200.026.99771510-14
Produced by FastQC (version 0.11.5)