FastQCFastQC Report
Sat 18 Mar 2023
SRR4895488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231145
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA1232300.0143.778271
CGAGTAA1232650.0143.737432
GAGTAAT434150.0142.957083
GAGTAAG397300.0142.831683
GAGTAAC112150.0142.37853
GAGTAAA301550.0140.601443
AGTAATA127450.0139.34514
AGTAATG151550.0139.265154
AGTAAGG144050.0139.072834
AGTAAGA113300.0138.583394
AGTAAGT123250.0137.963154
AGTAACG35450.0136.813834
AGTAATT128750.0136.708514
AGTAACT25850.0135.567254
AGTAAGC29650.0135.181384
GTAATAG44950.0134.953525
GTAATGA43650.0134.851385
GTAAGAG39150.0133.809195
AGTAATC41900.0133.613984
AGTAACC31300.0133.572854
Produced by FastQC (version 0.11.5)