FastQCFastQC Report
Sat 18 Mar 2023
SRR4895488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231145
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103890.843848612470505No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA16300.1323970775172705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15470.1256553858400107No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC14070.11428385770969302No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC13750.11168465127990611No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA13590.11038504806501265No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT12910.10486173440171548No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT12360.10039434835051922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151200.0137.050748
TGGTATC152500.0135.740847
GTGGTAT152400.0135.640996
AAGCAGT153000.0135.010941
GTATCAA154400.0134.070459
AGTGGTA155150.0133.283175
CAGTGGT155750.0132.769734
AGCAGTG158600.0130.289262
GCAGTGG161950.0127.638673
TCAACGC151200.028.03851510-14
ATCAACG151550.027.94526510-14
AGAGTAC151700.027.87086515-19
TACATGG153400.027.51887320-24
CAGAGTA153700.027.508215-19
GTACATG154100.027.4312420-24
CAACGCA154950.027.40639710-14
AGTACAT155000.027.24409720-24
GAGTACA151850.027.08865720-24
CGCAGAG156550.027.0074115-19
AACGCAG157800.026.88404510-14
Produced by FastQC (version 0.11.5)