FastQCFastQC Report
Sat 18 Mar 2023
SRR4895487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213853
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC2218050.0143.63152
GAGATCG678550.0143.09813
GAGATCT723000.0143.068043
AAGAGAT2226900.0143.060681
GAGATCA579650.0143.05993
AGATCGT244850.0142.140824
AGATCTG216150.0142.139744
AGATCTA202650.0142.093224
AGATCGA191550.0141.87564
GAGATCC247850.0141.697683
AGATCAT204250.0141.438064
AGATCAG180400.0141.31154
GATCGTA73050.0140.752085
AGATCAA170800.0140.154744
GATCGAT68350.0140.0095
AGATCGG196900.0139.77474
AGATCCG60500.0139.503634
GATCATG67700.0139.227655
GATCTAG64950.0138.918875
GATCGTG78900.0138.797135
Produced by FastQC (version 0.11.5)