FastQCFastQC Report
Sat 18 Mar 2023
SRR4895487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213853
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA225751.0197154011580716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39310.1775637316479459No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30900.13957566288276593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT30120.13605239372261846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA252350.0136.92638
GTGGTAT256100.0135.061846
TGGTATC256250.0134.92667
AAGCAGT257450.0134.486081
GTATCAA257050.0134.47879
AGTGGTA262650.0131.501855
CAGTGGT263250.0131.420844
AGCAGTG265150.0130.553422
GCAGTGG270450.0127.88853
TCAACGC253300.028.02130110-14
ATCAACG252900.027.9233310-14
AGAGTAC254850.027.75598515-19
CAGAGTA256800.027.51719715-19
TACATGG257300.027.4522720-24
GTACATG258850.027.32125920-24
CGTGGTA1856.1611427E-627.232335
GAGTACA254750.027.07712220-24
AGTACAT261900.026.88763820-24
CAACGCA264200.026.88158210-14
GCAGAGT263400.026.82769815-19
Produced by FastQC (version 0.11.5)