FastQCFastQC Report
Sat 18 Mar 2023
SRR4895486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237260
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC3770.158897412121723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG100.007247527143.920978
CGACTGG100.007247527143.920978
CGATCGG151.2659442E-4143.920968
AACTCAC237800.0143.58811
ACTCACC238150.0143.377082
CTCACCG93500.0142.99743
CTCACCT68650.0142.977573
TCACCTG25150.0142.776474
TCACCGA21800.0142.27054
TCACCGT30350.0141.787054
TCACCGG34850.0141.649634
CTCACCA43750.0141.289263
TCACCTA20550.0140.769424
CACCGGT14050.0139.823585
CACCGTG10050.0139.624825
ACCGGTA4500.0139.12366
CACCGTA7700.0138.313665
TCACCAG16700.0137.888344
CCGATTA1100.0137.37917
TCACCTT19450.0137.261384
Produced by FastQC (version 0.11.5)